REVISTA ECIPERU 14 (2017) 79-86

Nucleic acid detection using a universal electrochemical sensor for SNS differentiation

Detección de ácidos nucleicos usando un sensor electroquímico universal para la discriminación de SNS

Percy Calvo-Marzal1, Dmitry M. Kolpashchikov1,2,3 and Karin Y. Chumbimuni-Torres1

1 Department of Chemistry, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816

2 National Center for Forensic Science, University of Central Florida, Orlando, FL 32816

3 Burnett School of Biomedical Science, University of Central Florida, Orlando, FL 32816

Recibido el 26 de diciembre del 2017, aceptado el 31 de diciembre del 2017



Nucleic acid detection with high sensitivity and selectivity capabilities could aid in the diagnosis of varies diseases such as: infections, cancer, among others. Discrimination of a mismatch or single nucleotide substitution (SNS) is challenge due to a minimum pair to pair formation double strand needed to stabilize the structure. Here we present a new approach to atinge the needed stability by using additional segments strains to favored stabilization. The use of additional complementary strains facilitates the discrimination of a SNS in a cooperative way.

Keywords: Nuclei acid, Single nucleotide substitution, infection, cancer

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