Nucleic acid detection using a universal electrochemical sensor for SNS differentiation
Detección de ácidos nucleicos usando un sensor electroquímico universal para la discriminación de SNS
Percy Calvo-Marzal1, Dmitry M. Kolpashchikov1,2,3 and Karin Y. Chumbimuni-Torres1
1 Department of Chemistry, University of Central Florida, 4000 Central Florida Blvd., Orlando, FL 32816
2 National Center for Forensic Science, University of Central Florida, Orlando, FL 32816
3 Burnett School of Biomedical Science, University of Central Florida, Orlando, FL 32816
Recibido el 26 de diciembre del 2017, aceptado el 31 de diciembre del 2017
DOI: https://doi.org/10.33017/RevECIPeru2017.0008/
Abstract
Nucleic acid detection with high sensitivity and selectivity capabilities could aid in the diagnosis of varies diseases such as: infections, cancer, among others. Discrimination of a mismatch or single nucleotide substitution (SNS) is challenge due to a minimum pair to pair formation double strand needed to stabilize the structure. Here we present a new approach to atinge the needed stability by using additional segments strains to favored stabilization. The use of additional complementary strains facilitates the discrimination of a SNS in a cooperative way.
Keywords: Nuclei acid, Single nucleotide substitution, infection, cancer